Fluid Shifts (FS) Baseline Imaging Operations: With guidance from the ground teams, crewmembers continued supporting the second week of FS operations by conducting an Ultrasound 2 scan and eye exams using the Optical Coherence Tomography (OCT) hardware. Fluids Shift is divided into three one-week segments: Dilution Measures, Baseline Imaging, and Baseline Imaging using the Russian Chibis Lower Body Negative Pressure (LBNP) device. The experiment measures how much fluid shifts from the lower body to the upper body, in or out of cells and blood vessels, and determines the impact these shifts have on fluid pressure in the head, changes in vision and eye structures. Cell Mechanosensing- 3 (CMS-3) Closeout: Following completion of microscope observations last week, the crew completed closeout activities by removing the Thermal Container from the Microscope in Multi-purpose Small Payload Rack Work Volume (MSPR WV). The investigation identifies gravity sensors in skeletal muscle cells to develop countermeasures to muscle atrophy. Scientists believe that the lack of mechanical stress from gravity causes tension fluctuations in the plasma membrane of skeletal muscle cells which changes the expression of key proteins and genes and allows muscles to atrophy. Strata Payload Activation: The crew configured and activated Strata-1 in the US LAB. Strata-1 investigates the properties and behavior of regolith on small, airless bodies. Regolith is the impact-shattered “soil” found on asteroids, comets, the Moon, and other airless worlds, but it is different from soil on Earth in that it contains no living material. Strata-1’s goal is to provide answers about how regolith behaves and moves in microgravity, how easy or difficult it is to anchor a spacecraft in regolith, how it interacts with spacecraft and spacesuit materials, and other important properties. Mobile Servicing System (MSS) Operations/HTV-6 Get-ahead Tasks: Robotics Ground Controllers are currently performing 1A battery operations. The first bolts torque has been successfully released. Engineering and robotics teams are working a forward plan for battery 3A2-2 H1 bolt completion including increasing the maximum commanded torque. Additional attempts are tentatively scheduled tomorrow. Inadvertent Time Tag Command during 4A3 Battery Reconditioning: During 4A3 battery reconditioning operations this morning, a time-tagged command executed after the time tag queue had been cleared.The executed command was the Battery Charge Discharge Unit (BCDU) Fault Isolator Closed command. After the error was discovered, a BCDU Fault Isolator Open command was executed to correct. There were no impacts to the vehicle.Data will be evaluated following 4A3 battery reconditioning completion on May 4 to determine if there was any impact to the reconditioning effort. Human Research Facility (HRF) Resupply: The crew restocked HRF supplies and trashed expired and limited life items. Todays Planned Activities All activities were completed unless otherwise noted. CORRECTSIYA. Blood Collection r/g 2091 CORRECTSIYA. Venous blood sample processing using Plasma-03 centrifuge / r/g 2091 CORRECTSIYA. Sample Handover to USOS for MELFI Insertion / r/g 2091 RUEXP – Blood Sample Insertion into MELFI ENERGY Diet Log of Breakfast JEMAL Slide Table extension FLUID SHIFTS. OCT Setup FS-OCT-BL SETUP Baseline OCT Setup DRAGON Transfers Multi-Purpose Small Payload Rack (MSPR) Payload Closeout Ops CORRECTSIYA. Closeout Ops / r/g 2091 FINEMOTR Experiment Ops FLUID SHIFTS. Ultrasound 2 Power On FLUID SHIFTS Donning CardioLab Holter, Subject CORRECTSIYA. Questionnaire Photos / r/g 2091 FLUID SHIFTS Baseline Ultrasound Scan, Subject Formaldehyde Monitoring Kit (FMK) Stow Operations CIR Hardware Setup OTKLIK. Hardware Monitoring / r/g 1588 JEMRMS Hardware Checkout FLUID SHIFTS Scanning Operator Assistance SPHEROIDS Copying KUBIK 6 Data to EDR Intermodular TORU Test with Mated Progress 431 (DC1) r/g 2096 SPHEROIDS Taking KUBIK 6 Temperature Data SPHEROIDS Copying KUBIK 6 Data to EDR Medical Kit Audit / r/g 2056 Changeout of Replaceable Condensate Removal Lines  r/g 2056 Retracting Slide Table in JEMAL and closing Outer Hatch JEMRMS Equipment deactivation FLUID SHIFTS CCFP Baseline Test, Subject METERON Onboard Training Laser Eye Protection Disk Removal from Cupola windows Part 2 Periodic Fitness Evaluation – Nom Ops (subject) FLUID SHIFTS Assisted Test, Subject FLUID SHIFTS Baseline Test, Operator FLUID SHIFTS DPOAE Baseline Test, Subject FLUID SHIFTS Hardware Power Off and Stow FLUID SHIFTS Tonometry Baseline Setup FLUID SHIFTS. Ultrasound 2 Hardware Power Off FLUID SHIFTS Baseline Test, Operator FLUID SHIFTS Tonometer Test, Subject FLUID SHIFTS Doffing CardioLab Holter, Subject FLUID SHIFTS. Tonometry Equipment stow FLUID SHIFTS CCFP Baseline Stowage RSS1 laptop BRI data download / r/g 2052 DRAGON Transfers THERM Closeout Ops ENERGY Diet Log of Lunch SPHEROIDS. Temperature Check. URAGAN.  R&R and Test Photography r/g 2095 Changeout of Replaceable Condensate Removal Lines  r/g 2056 Pre-packing EDV 1240 shell for return / r/g 2093 WRS – Recycle Tank Fill from EDV Crew Prep For PAO PCO2M Interface Check PAO Snapchat [Deferred] Maintenance Water Recovery System (WRS): Payload Water Reservoir (PWR) Fill 1 SmartCycler (SCYC) Battery Check HRF Blood collection setup Vacuum cleaning ventilation grille on FGB interior panels (201, 301, 401) IMS Delta File Prep WRS-PWR-Reservoir Fill (PWR) DRAGON Transfers HRF2 Supply Kit Resupply Dragon Cargo Operations Conference ENERGY Diet Log of Dinner Completed Task List Items None Ground Activities All activities were completed unless otherwise noted. 1A battery bolt ops Nominal ground commanding Three-Day Look Ahead: Friday, 04/29: SmartCycler, METERON, ENERGY, MSPR Microscope closeout, N1 reconfig Saturday, 04/30: Crew off duty; housekeeping Sunday, 05/01: Crew off duty QUICK ISS Status Environmental Control Group: Component Status Elektron On Vozdukh Manual  1 SM Air Conditioner System (SKV1) On  2 SM Air Conditioner System (SKV2) Off Carbon Dioxide Removal Assembly (CDRA) Lab Standby Carbon Dioxide Removal Assembly (CDRA) Node 3 Operate Major Constituent Analyzer (MCA) Lab Idle Major Constituent Analyzer (MCA) Node 3 Operate Oxygen Generation Assembly (OGA) Process Urine Processing Assembly (UPA) Normal Trace Contaminant Control System (TCCS) Lab Off Trace Contaminant Control System (TCCS) Node 3 Full Up Read full story.