Microscopy images can be used for detection of compelling forms of biosignature data, but due to limited sample throughput, fragility, and uncertainty in data analysis microscopes have been an instrument seldomly used for lander missions. Our digital holographic microscope (DHM) allows for high throughput and the robustness needed for space missions. Computer algorithms are now able to distinguish microbial motility from abiotic motion giving us the ability to automate the analysis of these images. Increasing the observation of motility biosignatures can be done by adding a stimulus to their environment. Electrical stimulus has been shown to alter the behavior of electroactive motile microbes in 2D. Using our DHM we are learning under what conditions an electrode can be used to stimulate and alter 3D motion of our test organism Shewanella oneidensis MR1. This organism is a facultative anaerobe that has been discovered to have the ability to transport electrons extracellularly to a terminal electron acceptor through a variety of pathways. Development of a sample chamber with a translucent ITO film electrode has allowed us to observe these organisms in 3D with negligible noise from the electrode. This electrostimulation method increased the number of motile microbes within the field of view and increased the speed at which they are swimming. Further analysis is underway to quantify these metrics. Understanding how external insoluble electron acceptors impact bacterial behavior and metabolism play a role in areas of research ranging from astrobiology life detection to global biogeochemical cycling processes.